All potential hazards must be taken into account while conducting experiments in this facility and access is only granted upon corresponding training. Radioactive labelling is conducted in SU and is restricted to Radioisotope room which is interconnected with the Dark room (Room FENS 2078). For information on disposal of other hazardous materials please see Waste Management section. This eliminates the need to collect the gels as a hazardous waste. Gels containing ethidium bromide can be easily de-stained in the laboratory by simply placing the gels in a DI-water bath for 15 minutes and gently agitating or 15 min treatment under UV light. Įthidium Bromide waste in concentrated or solid form is collected as hazardous waste and should not be flushed down the drain or disposed of in the trash. For more information see Emergency Procedures. A solution of soap and water is recommended for cleaning small spills and removing residues on equipment and laboratory surfaces. During normal use, small spills may occur and residues may build up on equipment and other laboratory surfaces.Wear insulated gloves and point the flask opening away from you. Let hot agarose solutions cool to 50-60 ☌ before adding ethidium bromide or pouring into trays. Exercise caution when using a microwave to melt agarose solutions don’t use sealed containers, and beware of superheated liquids that may suddenly and unexpectedly boil. ![]() Consider using ethidium bromide substitutes.Purchase pre-made gels or pre-mixed acrylamide and ethidium bromide solutions instead of making your own.Measure, mix, and handle hazardous powdered chemicals or gel prep mixtures with hazardous components (e.g., acrylamide monomer, phenol, ammonium persulfate, and formaldehyde) in the fume hood.Training should include special hazards and safety precautions. Responsible Faculty Member or LS are responsible for ensuring that all users are trained to use the equipment in a safe manner. Only trained and qualified users are permitted to operate gel electrophoresis equipment.Locations where ethidium bromide is used or stored must be identified with "Mutagenic" marked stickers.Use double gloves while working in the gel room, remove the contaminated gloves safely and dispose of before leaving the room. ![]() Instructions should include operating procedures written by the manufacturer and laboratory, as well as the associated hazards, the correct personal protective equipment (e.g., lab coats, gloves, and eye protection), and applicable emergency procedures.Read and follow manufacturer’s instructions for electrophoresis equipment.Acrylamide – carcinogen, neurotoxin, irritant.Hazardous chemicals commonly used in conjunction with electrophoresis work include: Ultraviolet (UV) light boxes and handheld lamps are often used in visualizing ethidium bromide gels and pose potential exposures to UV radiation. Users may be exposed to thermal hazards when heating agarose solutions. Switch off all power supplies and unplug the leads before opening the gel chamber lid or reaching inside the gel chamber.Gel chamber exterior must be dry with no spilled solutions.Gel chamber must have a lid or cover with safety interlocks to prevent accidental contact with energized electrodes or buffer solutions.Keep equipment clear of unintentional grounding points and conductors (e.g., sinks or other water sources, metal plates, jewellery, aluminium foil, pipes or other electrical/metal equipment). ![]()
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